SHH, Mouse, Recombinant

05PR00084

产品信息 产品形态 粉末 产品规格 10μg / 50μg / 500μg / 1mg 别名 Sonic Hedgehog Protein;SHH;HHG-1;SHH 物种 小鼠 表达宿主 E.coli 序列信息 Cys25-Gly198 检索号 Q62226 分子量 19.8 kDa 表观分子量 20 kDa 纯度 > 95% as determined by reducing SDS-PAGE. 内毒素 < 0.1 EU/μg 储存条件 Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. 运输条件 Ambient temperature or ice pack. 有效期 12个月 制剂 Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. 复融 Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 体系描述 GMP生产和质量体系 公司符合cGMP的要求，已通过NMPA的第一类医疗器械生产备案，且通过ISO 13485和ISO 9001标准认证。公司生产的SHH, Mouse, Recombinant符合ISO 9001的cGMP标准。 背景介绍 Mouse Sonic Hedgehog Homolog (SHH) belongs to a three-Protein family called Hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog Proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases.Mouse Shh is synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region. The mature region is autocatalytically processed into a nonglycosylated, 20 kDa, 174 aa N-terminal fragment (Shh-N), and a catalytic-processing,glycosylated, 34 kDa, 239 aa C-terminal fragment. The 20 kDa Shh-N fragment is the core of the active hedgehog molecule. Mouse Shh-N is 99%, 98%, and 100% aa identical to human, rat and gerbil Shh-N, respectively.