EGFR/ErbB1/HER1 (C-6His), Human, Recombinant

05PR00065

产品信息 产品形态 粉末 产品规格 10μg / 50μg / 500μg / 1mg 别名 Epidermal Growth Factor Receptor;Proto-oncogene c-ErbB-1;Receptor tyrosine- Protein kinase erbB-1;EGFR;ERBB;ERBB1;HER1 物种 人 表达宿主 Human Cells 序列信息 Leu25-Ser645 检索号 P00533 分子量 69.6 kDa 表观分子量 90-120 kDa 标签 C-6His 生物活性 Loaded Anti-Human EGFR mAb-Fc on Pro A Biosensor, can bind Recombinant Human EGFR-His with an affinity constant of 7.23 nM as determined in BLI assay. 纯度 > 95% as determined by reducing SDS-PAGE. 内毒素 < 0.1 EU/μg 储存条件 Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. 运输条件 Ambient temperature or ice pack. 有效期 12个月 制剂 Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 8% Sucrose, 50 mM Nacl, 0.05% Tween80, pH 8.0. 复融 Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 体系描述 GMP生产和质量体系 公司符合cGMP的要求，已通过NMPA的第一类医疗器械生产备案，且通过ISO 13485和ISO 9001标准认证。公司生产的EGFR/ErbB1/HER1 (C-6His), Human, Recombinant符合ISO 9001的cGMP标准。 背景介绍 The EGFR subfamily of Receptor tyrosine kinases is composed of EGFR, ErbB2, ErbB3 and ErbB4. The EGFR shares 43%-44% aa sequence identity with the ECD of human EGFR subfamily. All these family members are type I transmembrane glyco Proteins with an extracellular Ligand binding domain. The extracellular Ligand binding domain is containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain. Ligand binding could induce EGFR homodimerization and heterodimerization with ErbB2, resulting in cell signaling, heterodimerization tyrosine phosphorylation and kinase activation. It can bind EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, epigen, and so on. Its signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis. EGFR can also be recruited to form heterodimers with the Ligand-activated ErbB3 or ErbB4. EGFR is overexpressed in different tumors. Several anti-cancer drugs use EGFR as target.