DMS 114（人肺癌细胞）

05PR00035

产品信息 产品名称 DMS 114（人肺癌细胞） 别称 DMS-114;DMS114;Darmouth Medical School 114 RRID CVCL_1174 种属 人，白人 生长特性 贴壁细胞 细胞形态 上皮细胞样 冻存条件 冻存液： 无血清非程序冻存液[PB180438] 温度：液氮 培养方案A（默认） 生长培养基： Waymouth's MB 752/1 [PM151215] + 10% FBS [164210] + 1% P/S [PB180120] 培养条件：气相：空气，95%；CO₂，5%, 温度：37℃ 推荐传代比例 1:2-1:4 推荐换液频率 2-3次/周 参考资料(来源文献) 背景描述 The line was established from cells from a mediastinal biopsy of a patient with small cell carcinoma of the lung. The patient had not received prior therapy.The patient had not received prior therapy. The cells express HLA class I and class II antigens. Early passages of the cells were contaminated with a bovine mycoplasma (Acholeplasma laidlawii) which was cured (prior to cryopreservation) with A. laidlawii antiserum and kanamycin. 年龄（性别） 68Y;Male 组织来源 肺 细胞类型 肿瘤细胞 肿瘤类型 肺癌细胞 生物安全等级 BSL-1 抗原表达情况 Leu 7 +; My23 +; CD11b + 基因表达情况 adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; glucagon; 17 beta estradiol; oxytocin - neurophysin (OT-NP) 致瘤性 Yes; Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells. 保藏机构 ATCC; CRL-2066