抗 MDM2 磁珠
02AB00099
MDM2 proto-oncogene (MDM2) is a critical oncoprotein primarily known for its role as a negative regulator of the tumor suppressor protein p53. Structurally, MDM2 features several functional domains, including a p53-binding domain, a RING finger domain that provides E3 ubiquitin ligase activity, and a zinc finger motif that facilitates various protein interactions. MDM2 inhibits p53 by binding to its transactivation domain, preventing p53 from activating genes responsible for cell cycle arrest and apoptosis, and promoting its ubiquitination and degradation via the proteasome. This interaction is crucial in the context of cancer, as MDM2 is often overexpressed in various malignancies such as sarcomas, gliomas, and breast cancers, leading to decreased p53 activity and contributing to tumorigenesis. Additionally, MDM2's ability to shuttle p53 from the nucleus to the cytoplasm further enhances p53 degradation. The intricate regulation of MDM2 and p53 forms a feedback loop that is vital for maintaining cellular homeostasis; however, dysregulation of this loop is implicated in cancer progression.
| 规格 | 起售价 |
|---|---|
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技术参数
- 宿主
- Mouse
- 靶点基因名
- MDM2
- 蛋白别名
- MDM2 proto-oncogene; MDM2
- UniProt
- Q00987
- 物种
- Human
- 应用
- IP
产品详情
- 靶点名称
- MDM2
- 蛋白名称
- MDM2 proto-oncogene
- 蛋白别名
- E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein; Hdm2; Oncoprotein Mdm2; RING-type E3 ubiquitin transferase Mdm2; p53-binding protein Mdm2
- 靶点基因名
- MDM2
- UniProt
- Q00987
- 来源物种
- Homo sapiens (Human)
- 宿主物种
- Mouse
- 物种反应性
- Human
- 克隆性
- Monoclonal
- 亚型
- IgG1
- 配方
- Supplied as solution in phosphate buffered saline containing 0.1% BSA and 0.02% sodium azide
- 剂型
- Subject to solid-liquid separation upon standing; Mix thoroughly to ensure a consistent, homogeneous
免疫原核心序列
KPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYS
| 应用 | 推荐稀释比 | 备注 |
|---|---|---|
| IP | — | 50-100 μL/200 μg cell lysate |
背景介绍
MDM2 proto-oncogene (MDM2) is a critical oncoprotein primarily known for its role as a negative regulator of the tumor suppressor protein p53. Structurally, MDM2 features several functional domains, including a p53-binding domain, a RING finger domain that provides E3 ubiquitin ligase activity, and a zinc finger motif that facilitates various protein interactions. MDM2 inhibits p53 by binding to its transactivation domain, preventing p53 from activating genes responsible for cell cycle arrest and apoptosis, and promoting its ubiquitination and degradation via the proteasome. This interaction is crucial in the context of cancer, as MDM2 is often overexpressed in various malignancies such as sarcomas, gliomas, and breast cancers, leading to decreased p53 activity and contributing to tumorigenesis. Additionally, MDM2's ability to shuttle p53 from the nucleus to the cytoplasm further enhances p53 degradation. The intricate regulation of MDM2 and p53 forms a feedback loop that is vital for maintaining cellular homeostasis; however, dysregulation of this loop is implicated in cancer progression.